What question did this study set out to answer?

To examine the mechanisms responsible for the elevated glycosaminoglycan concentrations observed in murine tumors in vivo compared to tumor cell output in vitro.

January 1, 1984Journal of Cellular Biochemistry

Stimulation of glycosaminoglycan production in murine tumors

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Authors

WKWarren KnudsonEast Carolina University CBChitra BiswasChittagong University of Engineering & Technology BTBryan P. TooleForest Institute

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Implication

Preclinical study demonstrates tumor extracts stimulate fibroblast hyaluronate synthesis in murine models, suggesting host-tumor interactions regulate glycosaminoglycan production.

Key Points

To examine the mechanisms responsible for the elevated glycosaminoglycan concentrations observed in murine tumors in vivo compared to tumor cell output in vitro.
Measured concentrations of glycosaminoglycan (GAG), hyaluronate, and chondroitin sulfates in murine B-16 melanoma, A-10 carcinoma, and S-180 sarcoma in vivo relative to normal muscle and subcutaneous tissue.
Assessed in vitro GAG synthesis via [3H] acetate incorporation and chemical analysis in tumor lines across varying culture conditions, cocultures with fibroblasts, and treatments with conditioned media or tumor extracts.
In vivo tumors exhibited elevated GAG concentrations; A-10 carcinoma contained approximately 6-fold more hyaluronate than subcutaneous tissue and 18-fold more than muscle tissue, with elevated chondroitin sulfates across all three tumors.
Cultured tumor cell lines synthesized less than 5% as much GAG as fibroblasts, an output unaffected by passage number, medium alterations, or hyaluronidase activity.
Tumor extracts, but not conditioned media or intact cell cocultures, stimulated hyaluronate synthesis by fibroblasts, with A-10 carcinoma extracts exceeding and acting additively with serum.

Cite This Study

Knudson et al. (1984) studied this question.

synapsesocial.com/papers/6a771fbdd3a6aa87c6bd9510 https://doi.org/10.1002/jcb.240250402

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Implication

Preclinical study demonstrates tumor extracts stimulate fibroblast hyaluronate synthesis in murine models, suggesting host-tumor interactions regulate glycosaminoglycan production.

Stimulation of glycosaminoglycan production in murine tumors

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Implication

Key Points

Cite This Study

Stimulation of glycosaminoglycan production in murine tumors

Discussion

Member takes

Implication

Key Points

Cite This Study

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