What question did this study set out to answer?

Identify the functional human receptor for CC chemokine ligand 18 (CCL18) to elucidate its mechanistic role in chronic inflammatory diseases.

September 2, 2013The Journal of Experimental MedicineOpen Access

Identification of human CCR8 as a CCL18 receptor

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Authors

SISabina A. IslamHarvard University MLMorris LingHarvard University JLJohn LeungRetina Specialists

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Implication

Laboratory study demonstrates that CCR8 functions as a CCL18 receptor in Th2 cells and eosinophilic esophagitis tissue, highlighting a key pathway in chronic human inflammation.

Key Points

Identify the functional human receptor for CC chemokine ligand 18 (CCL18) to elucidate its mechanistic role in chronic inflammatory diseases.
Evaluated chemotaxis, calcium flux, receptor binding affinity, and receptor internalization in CCR8-transfected cells and activated human Th2 cells stimulated with CCL18.
Assessed cell migration responses to CCL18 in wild-type compared with Ccr8-deficient activated mouse Th2 cells.
Measured CCL18 and CCR8 coexpression levels in esophageal biopsy tissue isolated from individuals with active eosinophilic esophagitis, patients in remission, and healthy controls.
CCL18 bound CCR8 with high affinity, competed with endogenous ligand CCL1, stimulated receptor internalization, and drove heterologous cross-desensitization and calcium flux.
CCL18 promoted directed chemotaxis in CCR8-transfected cells, human Th2 cells, and wild-type mouse Th2 cells, but failed to elicit migration in Ccr8-deficient mouse Th2 cells.
CCL18 and CCR8 were strongly coexpressed at markedly higher levels in biopsy samples from patients with active eosinophilic esophagitis compared to those in remission or normal controls.

Cite This Study

Islam et al. (2013) studied this question.

synapsesocial.com/papers/6a773162137c7dcc81bdac88 https://doi.org/10.1084/jem.20130240

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Implication

Laboratory study demonstrates that CCR8 functions as a CCL18 receptor in Th2 cells and eosinophilic esophagitis tissue, highlighting a key pathway in chronic human inflammation.

Identification of human CCR8 as a CCL18 receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification of human CCR8 as a CCL18 receptor

Discussion

Member takes

Implication

Key Points

Cite This Study

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