Key result
Long-term primary in vitro culture of porcine atrial cardiomyocytes increased expression of collagen-encoding genes (COL5A2, COL8A1, COL12A1) but downregulated cellular architecture genes.
Why the study?
A detailed molecular characterization of cells extracted from the myocardium is needed to better understand the molecular mechanisms of cardiovascular pathologies and develop effective therapeutic options.
In vitro culture of porcine atrial cardiomyocytes creates a favorable environment for extracellular matrix reconstruction but may be suboptimal for maintaining intracellular structural gene expression.
In vitro atrial cardiomyocyte profiles warrant cautious interpretation; leaves open relevance to in vivo CVD mechanisms.
Numerous cardiovascular diseases (CVD) eventually lead to severe myocardial dysfunction, which is the most common cause of death worldwide. A better understanding of underlying molecular mechanisms of cardiovascular pathologies seems to be crucial to develop effective therapeutic options. Therefore, a worthwhile endeavor is a detailed molecular characterization of cells extracted from the myocardium. A transcriptomic profile of atrial cardiomyocytes during long-term primary cell culture revealed the expression patterns depending on the duration of the culture and the heart segment of origin (right atrial appendage and right atrium). Differentially expressed genes (DEGs) were classified as involved in ontological groups such as: “cellular component assembly”, “cellular component organization”, “cellular component biogenesis”, and “cytoskeleton organization”. Transcriptomic profiling allowed us to indicate the increased expression of COL5A2, COL8A1, and COL12A1, encoding different collagen subunits, pivotal in cardiac extracellular matrix (ECM) structure. Conversely, genes important for cellular architecture, such as ABLIM1, TMOD1, XIRP1, and PHACTR1, were downregulated during in vitro culture. The culture conditions may create a favorable environment for reconstruction of the ECM structures, whereas they may be suboptimal for expression of some pivotal transcripts responsible for the formation of intracellular structures.
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Nawrocki et al. (2022) studied Cardiovascular diseases (in vitro porcine model). Long-term primary in vitro cell culture was evaluated on Differentially expressed genes (DEGs) related to structural organization. Long-term primary in vitro culture of porcine atrial cardiomyocytes increased expression of collagen-encoding genes (COL5A2, COL8A1, COL12A1) but downregulated cellular architecture genes.
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