Isolation and Characterization of Tannins from Euphorbia hirta

The aim of the study is to isolate and characterize tannins from Euphorbia hirta using various analytical techniques. Approximately 300g sample of E. hirta was extracted with 70% acetone, yielding 19mg of crude extract. Column chromatography and Thin Layer Chromatography (TLC) were employed to isolate and purify the tannin fractions. UV-Visible spectroscopy and Fourier Transform Infrared (FT-IR) analysis were used to quantify and elucidate the structure of the isolated tannins. The results showed a high total phenolic content of 43.47 mg TAE/g, with the presence of functional groups typical of polyphenols (OH, C-C,C=O,C-O,C-H). Although minor impurities were detected, the findings indicated a high amount of tannins present, sufficient for pharmaceutical formulations and drug development. Accurate structural determination and quantification of tannins facilitates reliable research and development, enabling in-depth studies of their pharmacological effects and potentially informing the creation of novel treatments.