Quantification of Tannin in Chromolaena Odorat (Kirinyu) Leaf Extract

Kirinyuh, also known as Chromolaena odorata, is one of the plants that can be utilized as a traditional remedy for the purpose of wound recovery.Tannins, specifically polyphenolic compounds obtained from plants and present in the stems, leaves, and epidermis, are present in these foliage parts.Young leaves are traditionally applied to wounds and crushed before being used to treat them.In fact, the medicinal properties of tannins are very broad including astringent, antimicrobial, antibacterial, antidiarrheal, anti-inflammatory, antioxidant effects, and tissue growth stimulation.Therefore, determining the tannin content in Chromolaena odorata is crucial in scientifically validating its traditional medicinal uses, ensuring quality control, and exploring new therapeutic applications.The objective of this study is to ascertain the tannin content in extracts of young and old leaves of the kirinyuh plant.In this reserach, kirinyuh leaves were extracted, using the soxhletation method and 96% ethanol as the solvent.The resulting extract was subsequently evaporated at 50℃ using a rotary evaporator.Tannin content was ascertained at a wavelength of 765 nm utilizing a standard gallic acid solution as a comparison in conjunction with a UV-Vis spectrophotometer.The findings of the study indicated that the mean tannin content of leaf extract from young kirinyuh plants was 0.036%, while that of leaf extract from old kirinyuh plants was 0.062%.