The Effect Of Kirinyuh Leaves Aqueous Extract (Chromolaena odorata (L) R.M.King & H.Rob.) on Phase 2 Collagen Density Wound Healing in Mice (Mus musculus)

Introduction: Various plants have been extensively researched for their ability to hasten wound healing. Kirinyuh plants (Chromolaena odorata (L) R.M.King H.Rob.) includes several secondary metabolite chemicals that can help to accelerate wound healing.Objective: To determine the effect of water extract of kirinyu leaves (C. odorata) on collagen density at the end of phase 2 wound healing in mice (Mus musculus) and the amount of concentration of water extract of kirinyu leaves (C. odorata) that is most effective in collagen formation.Methods: A total of 28 male mice aged 2-3 months were given an incision wound on the back and separated into four treatment groups by giving kirinyuh leaf aqueous extract (C. odorata). Group I received no therapy (control), Group II received 2.5% extract administration, Group III received 5% extract administration, and Group IV received 7.5% extract administration. Wound healing was observed histologically by measuring the density of collagen using Mallory's phosphotungstic acid hematoxylin stain solution with aniline blue. The ANOVA test was used statistically to examine variations in collagen density across groups.Results : The histology analysis revealed that the average quantity of collagen sequentially was 67.5% for group I, 59.5% for group II, 64.9% for group III, and 71.0% for group IV. Meanwhile, statistical tests revealed that there was no significant difference between the groups, with p0.05 significance.Conclusion: Administrating kirinyuh leaf aqueous extract (C. odorata) did not have a significant effect on the density of collagen in mice (Mus musculus).