What question did this study set out to answer?

This research aims to evaluate how activated macrophages produce nitric oxide and superoxide and their effects on killing Leishmania major.

March 1, 1994European Journal of Immunology

Production of nitric oxide and superoxide by activated macrophages and killing of Leishmania major

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This research aims to evaluate how activated macrophages produce nitric oxide and superoxide and their effects on killing Leishmania major.
Murine macrophages were activated using zymosan and interferon-gamma or lipopolysaccharide.
Cytotoxicity assays were performed to evaluate the killing of Leishmania major by macrophages.
The production of nitric oxide and superoxide was measured at various time points following activation.
Macrophages activated with zymosan and interferon-gamma produced both nitric oxide and superoxide, enhancing Leishmania major killing.
S-nitroso-acetyl-penicillamine and a compound releasing nitric oxide and superoxide showed high leishmanicidal activity.
Peroxynitrite did not induce cytotoxic effects, indicating that nitric oxide is essential for killing L. major.

Structured PICO

Population

Murine macrophages and Leishmania major parasites

Intervention

Activation with zymosan and interferon-gamma (ZYM/IFN-gamma), lipopolysaccharide (LPS) and IFN-gamma, or NO donors (S-nitroso-acetyl-penicillamine, 3-morpholino-sydnonimine hydrochloride)

Comparator

Zymosan alone, or untreated controls

Outcome

Production of nitric oxide (NO) and superoxide, and killing of Leishmania majorsurrogate

Macrophage-mediated killing of Leishmania major is dependent on the production of nitric oxide rather than superoxide or peroxynitrite.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

NO pathway merits investigation as leishmaniasis target; animal data leave open human translation.

Cite This Study

Assreuy et al. (1994) studied this question.

synapsesocial.com/papers/6a774fd3a0847c3df1ec6c58 https://doi.org/10.1002/eji.1830240328

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Production of nitric oxide and superoxide by activated macrophages and killing of Leishmania major

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Production of nitric oxide and superoxide by activated macrophages and killing of Leishmania major

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider