What question did this study set out to answer?

To determine the requirement of IFN consensus sequence binding protein (ICSBP/IRF8) in the development and functional maturation of plasmacytoid and myeloid dendritic cells.

February 1, 2003The Journal of Immunology

Cutting Edge: IFN Consensus Sequence Binding Protein/IFN Regulatory Factor 8 Drives the Development of Type I IFN-Producing Plasmacytoid Dendritic Cells

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Authors

HTHideki TsujimuraChiba Cancer Center TTTomohiko TamuraYokohama City University KOKeiko OzatoUniversity of Iowa

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Implication

Murine knockout study reveals that IRF8 governs plasmacytoid dendritic cell generation and functional maturation, highlighting an essential transcriptional regulator of innate immune defenses.

Key Points

To determine the requirement of IFN consensus sequence binding protein (ICSBP/IRF8) in the development and functional maturation of plasmacytoid and myeloid dendritic cells.
Analyzed dendritic cell subset composition and Toll-like receptor-stimulated maturation in ICSBP-deficient (ICSBP-/-) and wild-type mice.
Cultured bone marrow progenitor cells in an fms-like tyrosine kinase 3 ligand (Flt3L) system with or without ICSBP gene reintroduction.
Measured cytokine output, including IFN-alpha and IL-12p40, following Toll-like receptor pathway stimulation.
ICSBP-/- mice completely lacked B220(+)CD11b(-) plasmacytoid dendritic cells and generated B220(-)CD11b(+) myeloid dendritic cells that failed to mature upon Toll-like receptor activation.
ICSBP-/- bone marrow progenitors exhibited severe defects in generating plasmacytoid dendritic cells in Flt3L cultures, a defect reversed by reintroducing ICSBP.
Re-expression of ICSBP fully restored Toll-like receptor-induced maturation and reconstituted subset-specific IFN-alpha and IL-12p40 production to levels seen in wild-type controls.

Cite This Study

Tsujimura et al. (2003) studied this question.

synapsesocial.com/papers/6a7752367ecc675f86ae5ca7 https://doi.org/10.4049/jimmunol.170.3.1131

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Implication

Murine knockout study reveals that IRF8 governs plasmacytoid dendritic cell generation and functional maturation, highlighting an essential transcriptional regulator of innate immune defenses.

Cutting Edge: IFN Consensus Sequence Binding Protein/IFN Regulatory Factor 8 Drives the Development of Type I IFN-Producing Plasmacytoid Dendritic Cells

Discussion

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Implication

Key Points

Cite This Study

Cutting Edge: IFN Consensus Sequence Binding Protein/IFN Regulatory Factor 8 Drives the Development of Type I IFN-Producing Plasmacytoid Dendritic Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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