Key result
Dendroaspis natriuretic peptide (DNP) demonstrated significantly greater stability in rat plasma compared to ANP, BNP, and CNP, and was primarily degraded in the kidney by a metalloproteinase.
Dendroaspis natriuretic peptide exhibits superior plasma stability compared to other natriuretic peptides and is primarily degraded by renal metalloproteinases, suggesting potential therapeutic utility in cardiac diseases.
May inform stable natriuretic peptide development for cardiac disease; hypothesis-generating in animals and should not change practice.
Our previous study demonstrated that the concentration of dendroaspis natriuretic peptide (DNP) was markedly higher than that of atrial NP (ANP) in rabbit plasma, indicating that DNP has a different metabolic rate from other NPs. Therefore, the metabolic characteristics of DNP in mammals require further analysis. The stabilities of NPs were determined by incubating 125I‑labeled ANP, brain NP (BNP), C‑type NP (CNP) and DNP at 37˚C for 1, 2 and 4 h, and analyzing their profiles by reversed‑phase high‑performance liquid chromatography. 125I‑labeled ANP, BNP and CNP were quickly degraded in rat plasma, while 125I‑labeled DNP was stable for 4 h. The relative stability of the peptides following incubation in rat plasma followed the rank order of: DNP>>>ANP≥BNP>>CNP. Organs were also examined for the degradation of DNP, including the spleen, kidney, liver, heart and lung. The physiological target organ for the degradation of DNP was observed to be the kidney. Furthermore, degradation of DNP in the kidney was attenuated by phenanthroline, a metalloproteinase inhibitor. Therefore, these results indicate that DNP has a longer stability in plasma and that it may have strong therapeutic applications in cardiac disease.
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Kim et al. (2013) studied Healthy (animal model) (n=30). 125I-labeled DNP vs. 125I-labeled ANP, BNP, and CNP was evaluated on Degradation of 125I-labeled natriuretic peptides in rat plasma (p=<0.05). Dendroaspis natriuretic peptide (DNP) demonstrated significantly greater stability in rat plasma compared to ANP, BNP, and CNP, and was primarily degraded in the kidney by a metalloproteinase.
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