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October 15, 1984Biochemical Journal

The metabolism of neuropeptides. The hydrolysis of peptides, including enkephalins, tachykinins and their analogues, by endopeptidase-24.11

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Authors

RMRebecca MatsasPasteur Hellenic InstituteAKA J KennyUniversity of CopenhagenATAnthony J. TurnerUniversity of Leeds

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Implication

Randomized trial examines hydrolysis efficiency of neuropeptides by endopeptidase-24.11, suggesting functional specificity in enzyme activity.

Key Points

  • The study aims to understand how endopeptidase-24.11 hydrolyzes various neuropeptides and identifies specific interactions that affect efficiency.
  • Endopeptidase-24.11 was purified from pig kidney and tested against various neuropeptide substrates.
  • Hydrolysis efficiencies were measured using kinetic parameters like Km and kcat for different substrates.
  • Inhibition of endopeptidase-24.11 by various compounds was compared with pig kidney peptidyldipeptidase A.
  • Substance P was the most efficiently hydrolyzed substrate (Km = 31.9 microM; kcat. = 5062 min-1).
  • Kinetic studies revealed significant variation in efficiency based on substrate composition and site-specific interactions.
  • Phosphoramidon displayed greater inhibitory potency against endopeptidase-24.11 compared to synthetic inhibitors.

Cite This Study

Matsas et al. (1984) studied this question.

synapsesocial.com/papers/6a7757caa0847c3df1ec70cchttps://doi.org/10.1042/bj2230433
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Proteolysis in Neuropeptide Processing and Other Neural Functions1984 · 264 citations
  2. 2Biochemical and Immunological Properties of a Membrane‐Bound Brain Metalloendopeptidase: Comparison with Thermolysin‐Like Kidney Neutral Metalloendopeptidase1984 · 43 citations
  3. 3Substance P and [Leu]enkephalin are hydrolyzed by an enzyme in pig caudate synaptic membranes that is identical with the endopeptidase of kidney microvilli.1983 · 373 citations
  4. 4The purification and specificity of a neutral endopeptidase from rabbit kidney brush border1974 · 462 citations
  5. 5A monoclonal antibody to kidney endopeptidase-24.11. Its application in immunoadsorbent purification of the enzyme and immunofluorescent microscopy of kidney and intestine1983 · 72 citations