What question did this study set out to answer?

To characterize wild-type and mutant forms of glycogen synthase kinase-3 (GSK-3) and evaluate their physiological capacity to regulate endogenous glycogen synthase in intact cells.

September 17, 1996Proceedings of the National Academy of SciencesOpen Access

Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells.

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Authors

HEHagit Eldar-FinkelmanTel Aviv University GAGretchen ArgastRapt Therapeutics (United States)OFOrit FoordElectroimpact (United States)

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Implication

Experimental cell study demonstrates that constitutively active GSK-3 mutants inhibit glycogen synthase activity in intact 293 cells, indicating serine-9 is critical for regulating glycogen...

Key Points

To characterize wild-type and mutant forms of glycogen synthase kinase-3 (GSK-3) and evaluate their physiological capacity to regulate endogenous glycogen synthase in intact cells.
Expressed wild-type GSK-3 and specific mutants (S9A, S9E, and inactive K85,86MA) in a cell-free system to assess baseline kinase activity.
Transiently transfected 293 cells with wild-type and mutant GSK-3 constructs to measure relative intracellular kinase activity and changes in endogenous glycogen synthase activity.
Cells expressing the S9A mutant exhibited kinase activity 2.6-fold of control cells, compared to 1.8-fold for wild-type GSK-3 and 2.0-fold for the S9E mutant.
Endogenous glycogen synthase activity decreased by 50% in cells expressing the S9A mutant, while expression of wild-type GSK-3 and the S9E mutant suppressed activity by 20% and 30%, respectively.

Cite This Study

Eldar-Finkelman et al. (1996) studied this question.

synapsesocial.com/papers/6a7781547ecc675f86ae75dd https://doi.org/10.1073/pnas.93.19.10228

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Implication

Experimental cell study demonstrates that constitutively active GSK-3 mutants inhibit glycogen synthase activity in intact 293 cells, indicating serine-9 is critical for regulating glycogen...

Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

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