RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex.
Authors
Discussion
Loading...
Member takes
Implication
Molecular analysis reveals absence of I-J transcripts between I-A and I-E subregions in murine suppressor T cells, indicating alternative encoding.
Key Points
- To determine whether the genomic DNA region between the murine major histocompatibility complex I-A and I-E subregions encodes RNA transcripts for I-J polypeptides.
- Obtained cosmid clones from mouse sperm DNA spanning the entire genomic sequence between the I-A and I-E subregions.
- Hybridized the cloned DNA sequences to RNA prepared from multiple I-J-positive suppressor T-cell hybridomas and screened for genomic DNA rearrangements.
- Hybridization revealed no detectable RNA transcripts corresponding to the I-A to I-E intervening region in I-J-positive suppressor T-cell hybridomas.
- No DNA rearrangements were observed in the analyzed suppressor T cells, demonstrating that I-J polypeptides are not encoded between the I-A and I-E subregions.
Cite This Study
Kronenberg et al. (1983) studied this question.