What question did this study set out to answer?

To determine whether transplanting mesenchymal stem cells engineered to express insulin-like growth factor-I (MSCIGF) restores bone healing and overcomes nonunion fractures in mice.

July 26, 2011Stem CellsOpen Access

Mesenchymal Stem Cells Expressing Insulin-like Growth Factor-I (MSCIGF) Promote Fracture Healing and Restore New Bone Formation in Irs1 Knockout Mice: Analyses of MSCIGF Autocrine and Paracrine Regenerative Effects

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Authors

FGFroilán Granero‐MoltóNavarre Institute of Health Research TMTimothy J. MyersNational Cancer Institute JWJared A. WeisWake Forest University

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Implication

Preclinical study demonstrates that IGF-I-expressing mesenchymal stem cells enhance fracture healing and bone formation in mice, indicating therapeutic potential for nonunion fractures.

Key Points

To determine whether transplanting mesenchymal stem cells engineered to express insulin-like growth factor-I (MSCIGF) restores bone healing and overcomes nonunion fractures in mice.
Transplanted bone marrow-derived MSCIGF or traceable Lac-Z-tagged MSCIGF systemically into wild-type and insulin receptor substrate-1 knockout (Irs1-/-) mice with stabilized tibia fractures.
Evaluated bone healing using biomechanical testing, microcomputed tomography (μCT), and histological examinations.
Analyzed the molecular mechanisms governing autocrine and paracrine IGF-I actions and IRS1-PI3K signaling pathways both in vitro and in vivo.
Systemic MSCIGF transplantation improved fracture mechanical strength, accelerated mineralization, and increased new bone formation via combined autocrine and paracrine actions.
IGF-I triggered MSC differentiation into osteoblasts through an intact IRS1-PI3K signaling pathway.
Autocrine IGF-I signaling restored bone regeneration and established a well-organized bridging callus in Irs1-/- nonunion mice, which failed to heal when untreated or treated with unmodified MSCs.

Cite This Study

Granero‐Moltó et al. (2011) studied this question.

synapsesocial.com/papers/6a77880ee4645e92c9bd65a3 https://doi.org/10.1002/stem.697

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Implication

Preclinical study demonstrates that IGF-I-expressing mesenchymal stem cells enhance fracture healing and bone formation in mice, indicating therapeutic potential for nonunion fractures.

Mesenchymal Stem Cells Expressing Insulin-like Growth Factor-I (MSCIGF) Promote Fracture Healing and Restore New Bone Formation in Irs1 Knockout Mice: Analyses of MSCIGF Autocrine and Paracrine Regenerative Effects

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Key Points

Cite This Study

Mesenchymal Stem Cells Expressing Insulin-like Growth Factor-I (MSCIGF) Promote Fracture Healing and Restore New Bone Formation in Irs1 Knockout Mice: Analyses of MSCIGF Autocrine and Paracrine Regenerative Effects

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Cite This Study

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