Construction of N-[5-(Trifluoromethyl)-1H-indazol-3-yl]acetamide Derivatives; Their Anticancer Activity and Molecular Docking Interactions

A series of novel N-[5-(trifluoromethyl)-1H-indazol-3-yl]acetamide derivatives were synthesized starting from 2-hydroxy-5-(trifluoromethyl)benzonitrile. The starting compound was reacted with hydrazine hydrate to get 5-(trifluoromethyl)-1H-indazol-3-amine. Further reaction with chloroacetic anhydride to get 2-chloro-N-[5-(trifluoromethyl)-1H-indazol-3-yl]acetamide. This compound reacts with thiophenols or piperazines to afford designed ethyl amide-linked trifluoromethyl indazole hybrids. All the final compounds were evaluated for anticancer activity against four human cancer cell lines such as: HeLa-Cervical cancer (CCL-2), COLO 205-Colon cancer (CCL-222), HepG2-Liver cancer (HB-8065), MCF7-Breast cancer (HTB-22), and one normal cell line such as HEK-293 Human Embryonic Kidney cells (CRL-1573) and promising compounds have been identified. Molecular docking interactions were also evaluated.