What question did this study set out to answer?

To characterize the factor mediating multiplicity-dependent interference and investigate the conditions under which it is generated during respiratory syncytial virus infection.

August 1, 1982Infection and ImmunityOpen Access

Defective interfering particles of respiratory syncytial virus

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Authors

MTMary W. TreuhaftNorthwestern University MBMarc O. BeemUniversity of Chicago

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Implication

In vitro study demonstrates defective interfering particles inhibit replication in HEp-2 cells, suggesting a self-regulatory mechanism of viral infection.

Key Points

To characterize the factor mediating multiplicity-dependent interference and investigate the conditions under which it is generated during respiratory syncytial virus infection.
Cultured respiratory syncytial virus (Randall strain) and cloned viral stocks in HEp-2 cells.
Assayed the interfering factor using infectious yield reduction assays, UV irradiation inactivation, and challenge with heterologous vesicular stomatitis virus.
Assessed particle generation across serial undiluted viral passages compared to eight passages at a multiplicity of infection of 0.1.
The interfering factor was particulate, sensitive to UV inactivation, and homotypic, inhibiting cloned respiratory syncytial virus yield without suppressing vesicular stomatitis virus replication.
Defective interfering particles emerged after four undiluted passages of cloned virus, but were not detected after eight passages at a low multiplicity of infection of 0.1.

Cite This Study

Treuhaft et al. (1982) studied this question.

synapsesocial.com/papers/6a77916e7ecc675f86ae7fd2 https://doi.org/10.1128/iai.37.2.439-444.1982

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Implication

In vitro study demonstrates defective interfering particles inhibit replication in HEp-2 cells, suggesting a self-regulatory mechanism of viral infection.

Defective interfering particles of respiratory syncytial virus

Discussion

Member takes

Implication

Key Points

Cite This Study

Defective interfering particles of respiratory syncytial virus

Discussion

Member takes

Implication

Key Points

Cite This Study

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