What question did this study set out to answer?

To determine how anteroposterior neural patterning is established in Xenopus embryos and whether posterior neural identities can be derived from prospective anterior neural tissue.

December 1, 1995Development

Caudalization of neural fate by tissue recombination and bFGF

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Authors

WCWm. Gregory CoxRockefeller University AHAli Hemmati‐BrivanlouHoward Hughes Medical Institute

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Implication

Ex vivo explant study demonstrates that posterior axial tissue and bFGF caudalize anterior neural tissue in Xenopus embryos, indicating neural patterning occurs through progressive posteriorization.

Key Points

To determine how anteroposterior neural patterning is established in Xenopus embryos and whether posterior neural identities can be derived from prospective anterior neural tissue.
Generated tissue explants and recombinants combining early neural plate regions with posterior axial tissue from Xenopus embryos.
Tracked cell fate conversions across regional boundaries using lineage tracing and region-specific neural markers.
Tested the caudalizing capacity of basic fibroblast growth factor (bFGF) on prospective forebrain and naive ectodermal explants neuralized with follistatin and dominant-negative activin receptor.
Posterior axial tissue converted prospective forebrain explants into midbrain and hindbrain fates.
bFGF induced hindbrain markers in forebrain tissue, spinal cord fates in prospective hindbrain explants, and posterior neural fates in neuralized ectoderm without inducing mesoderm.
Forebrain competence to respond to posterior axial tissue and bFGF was restricted to early stages and was completely lost by the neural groove stage.

Cite This Study

Cox et al. (1995) studied this question.

synapsesocial.com/papers/6a7798247d04941475ae5bfd https://doi.org/10.1242/dev.121.12.4349

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Implication

Ex vivo explant study demonstrates that posterior axial tissue and bFGF caudalize anterior neural tissue in Xenopus embryos, indicating neural patterning occurs through progressive posteriorization.

Caudalization of neural fate by tissue recombination and bFGF

Discussion

Member takes

Implication

Key Points

Cite This Study

Caudalization of neural fate by tissue recombination and bFGF

Discussion

Member takes

Implication

Key Points

Cite This Study

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