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Ionic dependence of regenerative responses of the embryonic cell mnembrane has been studied successively at each stage of development from the unfertilized egg to the differentiated striated muscle in the tadpole larva of the tunicate. The unfertilized egg cell itself showed a type of regenerative response dependent on both sodium and calcium ions, while the spike potentials exclusively dependent on calcium ions were elicited in the differentiated muscle cell.
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Miyazaki et al. (1972) studied this question.
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