What question did this study set out to answer?

To determine how interspecies recombination of the penA gene with commensal Neisseria species confers penicillin resistance in Neisseria meningitidis.

January 1, 1994Journal of BacteriologyOpen Access

Interspecies recombination between the penA genes of Neisseria meningitidis and commensal Neisseria species during the emergence of penicillin resistance in N. meningitidis: natural events and laboratory simulation

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Authors

LBLucas D. BowlerBrighton and Sussex Medical School QZQ Y ZhangUniversity of Sussex JRJulien RiouStatens Serum Institut

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Implication

Laboratory transformation study reveals interspecies penA recombination drives penicillin resistance in Neisseria meningitidis, indicating natural exchange with commensal species.

Key Points

To determine how interspecies recombination of the penA gene with commensal Neisseria species confers penicillin resistance in Neisseria meningitidis.
Transformed a penicillin-susceptible isolate of Neisseria meningitidis using chromosomal DNA from commensal Neisseria cinerea and Neisseria flavescens.
Assessed transformants for increased penicillin resistance and analyzed penA gene sequences, including codon alterations and mosaic structures, alongside four N. cinerea isolates.
Chromosomal DNA from both N. cinerea and N. flavescens successfully transformed penicillin-susceptible N. meningitidis to increased penicillin resistance.
Resistance transformation via N. flavescens DNA directly introduced the donor penA gene, confirming that interspecies recombination mimics natural resistance development.
Transformants generated with N. cinerea DNA did not incorporate the donor penA gene, but an N. cinerea-derived penA segment in strain K196 carried an Asp-345A insertion absent in tested N. cinerea isolates.

Cite This Study

Bowler et al. (1994) studied this question.

synapsesocial.com/papers/6a77c21fa7498e5110aed5ce https://doi.org/10.1128/jb.176.2.333-337.1994

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Implication

Laboratory transformation study reveals interspecies penA recombination drives penicillin resistance in Neisseria meningitidis, indicating natural exchange with commensal species.

Interspecies recombination between the penA genes of Neisseria meningitidis and commensal Neisseria species during the emergence of penicillin resistance in N. meningitidis: natural events and laboratory simulation

Discussion

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Implication

Key Points

Cite This Study

Interspecies recombination between the penA genes of Neisseria meningitidis and commensal Neisseria species during the emergence of penicillin resistance in N. meningitidis: natural events and laboratory simulation

Discussion

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Implication

Key Points

Cite This Study

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