Direct Readout of Protein–Protein Interactions by Mass Spectrometry from Protein–DNA Microarrays

Catching proteins: Proteins from a protein–DNA microarray can be directly detected by using MALDI mass spectrometry. Capture proteins are attached to silicon chips in a highly selective and reversible way by DNA-directed immobilization (see picture). The analyte proteins bound specifically to their partners on the microarray can be detected from cell lysates.