Profiling Kinase Activities by Using a Peptide Chip and Mass Spectrometry

Weighing in: Multiple kinases can be assayed simultaneously by using a method that combines peptide chips and MALDI-TOF mass spectrometry (MS). The method uses self-assembled monolayers that present a set of peptides that are each selective for a kinase. The kinase phosphorylates the peptide, changing its mass. MS analysis of the surface resolves each peptide and gives the activity of each kinase.