What question did this study set out to answer?

To determine whether renin and other renin-angiotensin system components are synthesized and localized locally within rat adrenal cortex cells independently of renal circulation.

September 1, 1996AJP Endocrinology and Metabolism

Presence of renin within intramitochondrial dense bodies of the rat adrenal cortex

View Full Paper

Ask AI

Bookmark

Population

Rats (adrenal glomerulosa cells)

Comparison

Bilateral nephrectomy and hemodialysis vs Control rats

Design

Preclinical

Follow-up

60 h

Authors

JPJörg PetersUniversitätsmedizin Greifswald BKBettina KränzlinHeidelberg University SSSamantha SchaefferUniversité de Tours

Discussion

Member takes

Implication

Indicates kidney-independent adrenal renin in rats; leaves open its role in human aldosterone regulation.

Key Points

To determine whether renin and other renin-angiotensin system components are synthesized and localized locally within rat adrenal cortex cells independently of renal circulation.
Assessed adult rats 60 hours after bilateral nephrectomy and hemodialysis compared with intact controls to eliminate circulating renal renin.
Used immunocytochemical and biochemical methods to isolate and measure renin, angiotensin-converting enzyme, and angiotensinogen in adrenal mitochondria and intramitochondrial dense bodies.
Circulating renin was cleared after nephrectomy, but intra-adrenal renin content increased from 2 ± 0.5 ng ANG I·mg⁻¹·h⁻¹ in controls to 25 ± 2 ng ANG I·mg⁻¹·h⁻¹ in anephric rats.
Renin was localized to intramitochondrial dense bodies, which increased in size, count, and renin activity from 2.5 ± 0.7 ng ANG I·mg⁻¹·h⁻¹ in controls to 43 ± 7 ng ANG I·mg⁻¹·h⁻¹ in anephric rats.
Angiotensin-converting enzyme and angiotensinogen co-localized within adrenal mitochondria and dense bodies, including giant dense bodies in anephric rats.

Structured PICO

Population

Rats (adrenal glomerulosa cells)

Intervention

Bilateral nephrectomy and hemodialysis

Comparator

Control rats

Outcome

Intra-adrenal renin content and localization of renin, ACE, and angiotensinogensurrogate

Demonstrates the existence of an intracellular renin-angiotensin system within the intramitochondrial dense bodies of rat adrenal glomerulosa cells.

Cite This Study

Peters et al. (1996) studied this question.

synapsesocial.com/papers/6a77df587ecc675f86aeb039 https://doi.org/10.1152/ajpendo.1996.271.3.e439

View Full Paper

Ask AI

Bookmark

Presence of renin within intramitochondrial dense bodies of the rat adrenal cortex

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Presence of renin within intramitochondrial dense bodies of the rat adrenal cortex

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider