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August 9, 2026Analytical and Bioanalytical ChemistryOpen Access

Combination of antibody-based screening methods for the identification and quantification of Legionella pneumophila in process water

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Authors

PSPhilipp StreichTechnical University of MunichJRJohannes RedwitzHealth and Safety AuthorityEHEdith HarbichInstitute of Medical Microbiology and Hygiene

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Overview

Randomized trial demonstrates rapid quantification of Legionella pneumophila in process water, indicating improved risk assessments.

Key Points

  • To develop a rapid method for identifying and quantifying Legionella pneumophila in process water and enhance risk assessment capabilities.
  • Applied a flow-based chemiluminescence sandwich microarray immunoassay using cryopreserved standards.
  • Combined immunomagnetic separation, flow cytometry, and microarray immunoassay for quantification and subtyping.
  • Tested on real process water samples containing complex mixtures of Legionella subtypes.
  • Achieved quantification of Legionella pneumophila down to 10^5 total cells per 100 mL.
  • Displayed diagnostic sensitivity and reduced total processing time for bacterial detection.
  • Enabled identification of the highly infectious Philadelphia subtype at lower concentrations.

Cite This Study

Streich et al. (2026) studied this question.

synapsesocial.com/papers/6a782cfa2e1896536c83fc81https://doi.org/10.1007/s00216-026-06720-x
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