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August 7, 2026Veterinary Medicine and ScienceOpen Access

Molecular and Serological Screening of West Nile and Usutu Virus in Birds and Humans: A Sentinel Study in Finland

Ruut Uusitalo Maija T. Suvanto Sanna Sainmaa Viktor Olander Roope Nykänen Essi M. Korhonen Anne J. Jääskeläinen

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Key Points

The study aims to develop sensitive molecular assays for detecting West Nile and Usutu viruses in birds and humans in Finland while assessing exposure through serology.
Developed real-time PCR assays for detecting WNV and USUV nucleic acids in bird and human samples.
Evaluated the assays for sensitivity using deceased bird and human serum samples.
Tested bird samples for anti-WNV antibodies using an immunofluorescence assay.
Real-time PCR assays exhibited high sensitivity with detection limits of 13.0 copies/reaction for WNV and 14.6 copies/reaction for USUV.
Screened 163 deceased birds and 337 human serum samples, finding no WNV or USUV nucleic acids.
Bird samples tested negative for WNV antibodies, indicating limited current exposure.

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Implication

Evaluates West Nile and Usutu virus detection in birds and humans, indicating the need for enhanced surveillance.

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Cite This Study

Uusitalo et al. (2026) studied this question.

synapsesocial.com/papers/6a782d5f2e1896536c840665 https://doi.org/10.1002/vms3.71125

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