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August 9, 2026BMC GenomicsOpen Access

A rapid one-step NGS-based system integrating microhaplotypes and SNPs for forensic identification

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Authors

LSLinyu ShiShanxi Medical UniversityJHJiaxuan HaoZhejiang Sci-Tech UniversityJXJingjing XuShanxi Medical University

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Implication

Randomized trial evaluates a rapid NGS-based system for identifying samples, suggesting improved forensic analysis capabilities.

Key Points

  • The study aims to develop a rapid, one-step NGS-based forensic identification system integrating microhaplotypes and SNPs.
  • Developed a one-step library preparation method for NGS within 2.5 hours.
  • Integrated 24 microhaplotypes, 80 SNPs, and a Y-Indel marker for comprehensive analysis.
  • Conducted forensic validation, including assessments on degraded DNA and formalin-fixed tissues.
  • Total discrimination power of 1–2.0265 × 10 –39 observed for the developed system.
  • Achieved 100% genotyping accuracy on formalin-fixed paraffin-embedded tissues compared to > 38% dropout with STRs.
  • Demonstrated high effectiveness on severely degraded DNA with likelihood ratios supporting suspect contribution > 2.26 × 10 15.

Cite This Study

Shi et al. (2026) studied this question.

synapsesocial.com/papers/6a782d5f2e1896536c8406cbhttps://doi.org/10.1186/s12864-026-13237-1
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Parallel sequencing of 170 STR and 132 SNP markers using the FGID forensic four-in-one DNA typing kit on the DNBSEQ-G99RS platform2024 · 2 citations
  2. 2A DNA typing panel of 201 genetic markers for degraded samples: development and validation.2024
  3. 3Development and validation of a 154-plex MPS STR panel for Illumina and Nanopore: a comprehensive tool for forensic genetic analysis2026
  4. 4Feasibility study of a single‐primer extension‐based microhaplotype NGS system2024 · 4 citations
  5. 5Variant Allele Characterization in STR Markers Using Next-Generation Sequencing2026