Key result
Massively parallel sequencing accurately quantified mutations in oral poliovirus vaccine samples, demonstrating perfect agreement with MAPREC results and enabling whole-genome mutation monitoring.
Population
Oral poliovirus vaccine (OPV) samples and reference preparations (type 3 OPV)
Comparison
Massively parallel sequencing using… vs Mutant analysis by PCR and restriction enzyme…
Design
Preclinical
Authors
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May advance vaccine QC monitoring; leaves open regulatory replacement of MAPREC pending further validation.
Absolute Event Rate: 0.687% vs 0.729%
Massively parallel sequencing provides a highly sensitive and accurate method for quantifying mutations in live viral vaccines, offering a potential replacement for MAPREC in quality control.
Неверов et al. (2010) studied Live viral vaccine quality control (Oral poliovirus vaccine). Massively parallel sequencing (MPS) vs. Mutant analysis by PCR and restriction enzyme cleavage (MAPREC) was evaluated on Quantification of 472-C mutants in US neurovirulence reference NC2. Massively parallel sequencing accurately quantified mutations in oral poliovirus vaccine samples, demonstrating perfect agreement with MAPREC results and enabling whole-genome mutation monitoring.
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