Key result
MALDI-TOF mass spectrometry imaging revealed distinct spatial distribution patterns of angiotensin II metabolites in mouse kidney sections, with Ang III localized in the medulla and Ang-(1-7) in the cortex.
Population
Mouse kidney sections (12 μm) and in vitro recombinant enzymes
Comparison
Incubation with 10-1,000 μmol/l Angiotensin II… vs Heat-treated kidney sections and specific enzyme…
Design
Preclinical
Authors
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Enables spatial mapping of renal angiotensin metabolites in mice; leaves open translation to human kidney physiology or disease.
MALDI-TOF mass spectrometry provides a novel, highly specific molecular imaging method to map the spatial distribution of angiotensin II processing enzymes in tissues.
Grobe et al. (2012) studied Angiotensin metabolism in kidney. MALDI-TOF mass spectrometry imaging vs. Heat-treated kidney sections was evaluated on Spatial distribution and activity of enzymes involved in Ang II processing. MALDI-TOF mass spectrometry imaging revealed distinct spatial distribution patterns of angiotensin II metabolites in mouse kidney sections, with Ang III localized in the medulla and Ang-(1-7) in the cortex.
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