Key result
Determination of thermodynamic parameters for CNG RNA duplexes and hairpins allows for more accurate prediction of the thermodynamic stabilities and structures of CNG RNAs.
May aid modeling of CNG repeat disorders; extends thermodynamic databases but leaves open cellular validation.
Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides composed of two to seven of CAG, CCG, CGG, and CUG repeats. The thermodynamic parameters of helix propagation correlated with the presence of multiple N-N mismatches within CNG RNA duplexes were also determined. Moreover, the total stability of CNG RNA hairpins, as well as the contribution of trinucleotide repeats placed only in the stem or loop regions, was evaluated. The improved thermodynamic parameters allow to predict much more accurately the thermodynamic stabilities and structures of CNG RNAs.
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Broda et al. (2005) studied Trinucleotide repeat expansion diseases. Oligoribonucleotides with CNG trinucleotide repeats was evaluated on Thermodynamic stabilities of RNA with CNG trinucleotide repeats. Determination of thermodynamic parameters for CNG RNA duplexes and hairpins allows for more accurate prediction of the thermodynamic stabilities and structures of CNG RNAs.
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