What question did this study set out to answer?

To describe in molecular detail the unitary interaction between myosin and actin during ATP-driven energy transduction and force generation in muscle.

July 18, 2007Proceedings of the National Academy of SciencesOpen Access

A closer look at energy transduction in muscle

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Key result

State change in the actomyosin complex appears to occur by attachment of a hydrophobic triplet of myosin to an actin conduit and its subsequent linear movement, driving force generation.

Authors

HOHirofumi OnishiTokyo Medical and Dental University MMManuel F. MoralesUniversidad de Las Palmas de Gran Canaria

Discussion

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Implication

No immediate clinical implications; leaves open validation of this actomyosin mechanism in cardiac models.

Key Points

To describe in molecular detail the unitary interaction between myosin and actin during ATP-driven energy transduction and force generation in muscle.
Synthesized biochemical data, mutational findings, and previous diffraction models to map the actomyosin interface.
Constructed a structural model of the weakly bound state formed upon initial contact between the myosin head and actin.
Extended existing rigor-state crystallographic models by adding previously unresolved residues to model the strongly bound state.
Transition between weakly and strongly bound states involves the binding of a hydrophobic triplet in myosin (Trp-546, Phe-547, and Pro-548) to an actin conduit.
The actin conduit provides a hydrophobic guiding rail consisting of Ile-341, Ile-345, Leu-349, and Phe-352 along which the myosin triplet moves linearly.
Conformational changes initiated at the actomyosin interface propagate throughout the myosin head to drive mechanical work against actin filaments.

Cite This Study

Onishi et al. (2007) studied this question. State change in the actomyosin complex appears to occur by attachment of a hydrophobic triplet of myosin to an actin conduit and its subsequent linear movement, driving force generation.

synapsesocial.com/papers/6a78d0586971db5bdd6736f2 https://doi.org/10.1073/pnas.0705525104

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A closer look at energy transduction in muscle

Discussion

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Implication

Key Points

Cite This Study

A closer look at energy transduction in muscle

Discussion

Member takes

Implication

Key Points

Cite This Study

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