What question did this study set out to answer?

Determine the complete primary structure of the Dictyostelium discoideum myosin heavy chain gene to evaluate structural and domain conservation relative to muscle myosins.

December 1, 1986Proceedings of the National Academy of SciencesOpen Access

Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.

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Population

Dictyostelium discoideum myosin heavy chain gene

Comparison

DNA sequence analysis and protein sequence… vs Nematode and mammalian muscle myosins

Design

Preclinical

Authors

HWHans M. WarrickStanford University ALArturo De LozanneThe University of Texas at Austin LLLeslie A. LeinwandBoston University

Discussion

Member takes

Implication

Supports cross-species myosin comparisons; leaves open relevance to human cardiac myosin function or disease.

Key Points

Determine the complete primary structure of the Dictyostelium discoideum myosin heavy chain gene to evaluate structural and domain conservation relative to muscle myosins.
Cloned the Dictyostelium discoideum myosin heavy chain gene and determined the full primary sequence via DNA sequence analysis.
Compared the deduced 2,116-amino acid sequence and structural repeat periodicities against nematode and mammalian muscle myosins.
Identified a 2,116-amino acid protein organized into a globular head with conserved functional subdomains and an alpha-helical rod tail, although certain muscle-specific head features were absent.
Detected structural periodicities in the tail region identical to muscle myosins, including a 196-residue repeat, a 28-residue charge repeat, and a 1,4 hydrophobic repeat associated with filament formation.

Structured PICO

Population

Dictyostelium discoideum myosin heavy chain gene

Intervention

DNA sequence analysis and protein sequence comparison

Comparator

Nematode and mammalian muscle myosins

Outcome

Conserved functional and structural domains and periodicities

The Dictyostelium myosin heavy chain shares conserved head domains and tail periodicities with muscle myosins, suggesting similar filament structures despite missing certain features.

Cite This Study

Warrick et al. (1986) studied this question.

synapsesocial.com/papers/6a78d05a6971db5bdd673700 https://doi.org/10.1073/pnas.83.24.9433

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Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider