What question did this study set out to answer?

To provide a comprehensive, practical guide evaluating biochemical, molecular biological, and genetic strategies for identifying protein-protein interactions and measuring quantitative binding affinities.

March 1, 1995Microbiological ReviewsOpen Access

Protein-protein interactions: methods for detection and analysis

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Authors

EPEric M. Phizicky SFStanley Fields

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Overview

Methodological review details biochemical, molecular, and genetic approaches to analyze protein binding and affinities, highlighting technical advantages and analytical challenges across...

Key Points

To provide a comprehensive, practical guide evaluating biochemical, molecular biological, and genetic strategies for identifying protein-protein interactions and measuring quantitative binding affinities.
Reviewed qualitative biochemical detection assays, including protein affinity chromatography, affinity blotting, coimmunoprecipitation, and chemical cross-linking.
Evaluated molecular and genetic screening strategies, including yeast two-hybrid systems, phage display, and extragenic suppressor mutant isolation.
Assessed quantitative equilibrium binding approaches, such as sedimentation, gel filtration, fluorescence-based assays, and surface plasmon resonance.
Identified distinct technical advantages, limitations, and potential experimental artifacts associated with in vitro and in vivo interaction mapping techniques.
Demonstrated multi-method application frameworks through three well-characterized domain models engaged in complex macromolecular interactions.

Cite This Study

Phizicky et al. (1995) studied this question.

synapsesocial.com/papers/6a799b9efb5b6b43000dbff3 https://doi.org/10.1128/mr.59.1.94-123.1995

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March 1, 1995Microbiological ReviewsOpen Access

Protein-protein interactions: methods for detection and analysis

View Full Paper

Ask AI

Bookmark

Authors

EPEric M. Phizicky SFStanley Fields

Discussion

Member takes

Overview

Methodological review details biochemical, molecular, and genetic approaches to analyze protein binding and affinities, highlighting technical advantages and analytical challenges across...

Key Points

To provide a comprehensive, practical guide evaluating biochemical, molecular biological, and genetic strategies for identifying protein-protein interactions and measuring quantitative binding affinities.
Reviewed qualitative biochemical detection assays, including protein affinity chromatography, affinity blotting, coimmunoprecipitation, and chemical cross-linking.
Evaluated molecular and genetic screening strategies, including yeast two-hybrid systems, phage display, and extragenic suppressor mutant isolation.
Assessed quantitative equilibrium binding approaches, such as sedimentation, gel filtration, fluorescence-based assays, and surface plasmon resonance.
Identified distinct technical advantages, limitations, and potential experimental artifacts associated with in vitro and in vivo interaction mapping techniques.
Demonstrated multi-method application frameworks through three well-characterized domain models engaged in complex macromolecular interactions.

Cite This Study

Phizicky et al. (1995) studied this question.

synapsesocial.com/papers/6a799b9efb5b6b43000dbff3 https://doi.org/10.1128/mr.59.1.94-123.1995

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