The effect of hydrodynamics on chemiluminescence (CL) emission is investigated for the tris(2,2‘-bipyridine)ruthenium(II/III) (Ru(bpy) 3 2+ / 3+ ) electrogenerated chemiluminescence (ECL) system in solution phase and immobilized in Nafion. The maximum CL emission is obtained at low linear velocities for slow reactions, at intermediate linear velocities for reactions with intermediate kinetics, and at high linear velocities for fast reactions. Relative kinetic information for a variety of analytes is acquired by comparing charge-coupled device (CCD) images to stopped-flow and flow injection analysis data obtained with a photomultiplier tube (PMT). CCD images are also utilized to observe reproducibility and homogeneity of different Nafion film preparations. The spatial resolution provided by the CCD allows a more comprehensive analysis of the effects of flow on observed CL reaction kinetics than is possible with a PMT. These studies yield important guidelines for application and optimization of CL detection in flow injection, liquid chromatography, and capillary electrophoresis.
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Shultz et al. (1996) studied this question.
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