Key result
Chronic restraint stress increases Angiotensin II potency in rat carotid through increased generation of PGI2 and H2O2 and activation of the Akt pathway.
Chronic restraint stress enhances angiotensin II-induced vascular contraction in rat carotids via increased PGI2 and H2O2 generation and Akt pathway activation.
Mechanistic pathways in stress-enhanced Ang II vasoconstriction identified in rats; leaves open human relevance and therapeutic targeting.
Objectives To investigate the mechanisms underlying the effects of chronic restraint stress on the vascular contractile response induced by angiotensin (Ang) II in rat carotid. Methods Concentration–response curves for AngII were obtained in endothelium-intact or endothelium-denuded carotid rings, in the absence or presence of SC-560 (COX-1 inhibitor), SC-236 (COX-2 inhibitor), wortmannin (PI3K-Akt inhibitor), ML171 (NOX-1 inhibitor), VAS2870 (NOX-4 inhibitor), tiron (O2− scavenger) or PEG-catalase (H2O2 scavenger). 6-ketoPGF1α, TXB2, O2− or H2O2 levels and superoxide dismutase and catalase activity or expression were also measured in rat carotid. Key findings Stress increased AngII potency in rat carotid. Muscular COX-1 or COX-2-derived metabolites negatively modulated AngII-induced contraction in control rat carotid. Endothelial COX-1 or COX-2-derived metabolites positively modulated AngII-induced contraction in stressed rat carotid. PI3K-Akt, NOX-1, NOX-4, O2− and H2O2 positively modulated AngII-induced contraction in stressed rat carotid. Stress increased 6-ketoPGF1α or H2O2 generation and reduced catalase activity in rat carotid. Protein expression of COX-1, NOX-4 or p-Akt was increased in stressed rat carotid. Conclusions Stress increases AngII potency in rat carotid by a mechanism that involves the increased generation of PGI2 and H2O2 and the activation of Akt pathway. Such mechanism could play a pathophysiological role in cardiovascular diseases correlated with stress.
No takes yet. Share an insight, caveat, or question.
Côco et al. (2016) studied this question. Chronic restraint stress vs. Control (unstressed) was evaluated on Angiotensin II potency in rat carotid. Chronic restraint stress increases Angiotensin II potency in rat carotid through increased generation of PGI2 and H2O2 and activation of the Akt pathway.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: