Reactivity of Isolated Arteries After 5-Week-Lasting Period of Intermittent Fasting Followed by the Return to Ad Libitum Regimen in Healthy Rats Fed With Normal and High-Fat Diet

Figures

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  Fig. 1. Effect of transient (5-week-lasting) intermittent fasting on weekly calorie intake, body weight and systolic blood pressure in Wistar-Kyoto rats fed with control or high-fat diet. Experimental groups: CTD – control diet administered continuously ad libitum; HFD – high-fat diet administered continuously ad libitum; CTDi – control diet with transient (5-week-lasting) intermittent fasting; HFDi – high-fat diet with transient (5-week-lasting) intermittent fasting. Symbols represent mean values ± SEM; n = 6–9. *p<0.05, **p<0.01, ***p<0.001 CTDi vs. CTD; +p<0.05, ++p<0.01, +++p<0.001 HFDi vs. HFD; #p<0.05 HFD vs. CTR.

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  Fig. 2. Effect of transient (5-week-lasting) intermittent fasting on acetylcholine-induced relaxant responses in thoracic aortas (with intact (PVAT(+)) or removed (PVAT(−)) perivascular adipose tissue) from Wistar-Kyoto rats fed with control (a) or high-fat (b) diet. Experimental groups: CTD – control diet administered continuously ad libitum; HFD – high-fat diet administered continuously ad libitum; CTDi – control diet with transient (5-week-lasting) intermittent fasting; HFDi – high-fat diet with transient (5-week-lasting) intermittent fasting. Symbols represent mean values ± SEM; n = 6–9. *p<0.05, **p<0.01 CTD PVAT(+) vs. CTD PVAT(−); +p<0.05, ++p<0.01, +++p<0.001 CTDi PVAT(+)/HFDi PVAT(+) vs. CTDi PVAT(−)/HFDi PVAT(−); #p<0.05, ##p<0.01 CTD PVAT(+)/HFD PVAT(+) vs. CTDi PVAT(+)/HFDi PVAT(+); §p<0.05 HFD PVAT(−) vs. HFDi PVAT(-).

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  Fig. 3. Effect of transient (5﷓week-lasting) intermittent fasting on noradrenaline - induced contractile responses in abdominal aortas (a, b), superior mesenteric arteries (c, d), small mesenteric arteries (e, f) and femoral arteries (g, h) (with intact (PVAT(+)) or removed (PVAT(−)) perivascular adipose tissue) from Wistar-Kyoto rats fed with control (a,c,e,g) or high-fat (b,d,f,h) diet. Experimental groups: CTD – control diet administered continuously ad libitum; HFD – high-fat diet administered continuously ad libitum; CTDi – control diet with transient (5-week-lasting) intermittent fasting; HFDi – high-fat diet with transient (5-week-lasting) intermittent fasting. Symbols represent mean values ± SEM; n = 6–18. *p<0.05, **p<0.01, ***p<0.001 CTD PVAT(+)/HFD PVAT(+) vs. CTD PVAT(−)/HFD PVAT(−); +p<0.05, ++p<0.01, +++p<0.001 CTDi PVAT(+)/HFDi PVAT(+) vs. CTDi PVAT(−)/HFDi PVAT(−); #p<0.05, ##p<0.01 CTD PVAT(+)/HFD PVAT(+) vs. CTDi PVAT(+)/HFDi PVAT(+); §p<0.05, §§p<0.01, §§§p<0.001 CTD PVAT(﷓)/HFD PVAT(−) vs. CTDi PVAT(−)/HFDi PVAT(−).

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  Fig. 4. Effect of transient (5-week-lasting) intermittent fasting on tyramine-induced contractile responses in abdominal aortas (a, b), superior mesenteric arteries (c, d), small mesenteric arteries (e, f) and femoral arteries (g, h) (with intact (PVAT(+)) or removed (PVAT(−)) perivascular adipose tissue) from Wistar-Kyoto rats fed with control (a,c,e,g) or high-fat (b,d,f,h) diet. Experimental groups: CTD – control diet administered continuously ad libitum; HFD – high-fat diet administered continuously ad libitum; CTDi – control diet with transient (5-week-lasting) intermittent fasting; HFDi – high-fat diet with transient (5-week-lasting) intermittent fasting. Symbols represent mean values ± SEM; n = 6–18. *p<0.05, **p<0.01, ***p<0.001 CTD PVAT(+)/HFD PVAT(+) vs. CTD PVAT(−)/HFD PVAT(−); +p<0.05, ++p<0.01, +++p<0.001 CTDi PVAT(+)/HFDi PVAT(+) vs. CTDi PVAT(−)/HFDi PVAT(−); #p<0.05, ##p<0.01, ###p<0.001 CTD PVAT(+)/HFD PVAT(+) vs. CTDi PVAT(+)/HFDi PVAT(+); §p<0.05, §§p<0.01 CTD PVAT(−)/HFD PVAT(−) vs. CTDi PVAT(−)/HFDi PVAT(−).