What question did this study set out to answer?

To characterize the nuclear role of the Dengue virus NS5 protein in altering cellular pre-mRNA splicing and determine how this interaction impacts viral replication.

August 30, 2016PLoS PathogensOpen Access

The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing

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Authors

FMFederico A. De MaioConsejo Nacional de Investigaciones Científicas y Técnicas GRGuillermo RissoConsejo Nacional de Investigaciones Científicas y Técnicas NINéstor Gabriel IglesiasConsejo Nacional de Investigaciones Científicas y Técnicas

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Implication

Experimental in vitro study reveals Dengue virus NS5 binding to spliceosome components in infected host cells, suggesting hijacked pre-mRNA processing promotes viral replication.

Key Points

To characterize the nuclear role of the Dengue virus NS5 protein in altering cellular pre-mRNA splicing and determine how this interaction impacts viral replication.
Conducted global proteomic analysis and functional interaction assays in infected cells to identify binding between NS5 and host spliceosome machinery.
Assessed endogenous and minigene alternative splicing events, host antiviral factor mRNA isoforms, and genome-wide intron retention using transcriptome sequencing.
Silenced specific U5 snRNP components, including CD2BP2 and DDX23, to test effects on pre-mRNA processing and Dengue viral replication.
NS5 interacted directly with core U5 snRNP components CD2BP2 and DDX23, altering alternative splicing patterns and changing mRNA isoform abundance of antiviral factors.
Transcriptome analysis demonstrated increased intron retention during infection, with NS5 reducing pre-mRNA processing efficiency independently of its enzymatic activities.
Targeted silencing of specific U5 snRNP components enhanced Dengue virus replication, indicating that spliceosome disruption creates a permissive viral environment.

Cite This Study

Maio et al. (2016) studied this question.

synapsesocial.com/papers/6a79cd615968508e56f3964e https://doi.org/10.1371/journal.ppat.1005841

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Implication

Experimental in vitro study reveals Dengue virus NS5 binding to spliceosome components in infected host cells, suggesting hijacked pre-mRNA processing promotes viral replication.

The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing

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Implication

Key Points

Cite This Study

The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing

Discussion

Member takes

Implication

Key Points

Cite This Study

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