July 1, 1993Genes & DevelopmentOpen Access

Conformational changes of U6 RNA during the spliceosome cycle: an intramolecular helix is essential both for initiating the U4-U6 interaction and for the first step of slicing.

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Population

U6 RNA, U4 RNA, U2 RNA, and spliceosome models

Design

Preclinical

Authors

TWThorsten WolffRobert Koch Institute ABAlbrecht BindereifJustus-Liebig-Universität Gießen

Discussion

Member takes

Implication

Highlights U6 stem II dynamics in models; leaves open relevance to human splicing disorders.

Structured PICO

Population

U6 RNA, U4 RNA, U2 RNA, and spliceosome models

Intervention

Mutational approach targeting the stem II region of U6 RNA (point mutations to alter stability of the intramolecular helix)

Outcome

U4-U6 base-pairing interaction and ability to support the first step of splicing

The stem II region of U6 RNA is essential for both initiating U4-U6 interaction and the first step of splicing, requiring balanced conformational stability.

Cite This Study

Wolff et al. (1993) studied this question.

synapsesocial.com/papers/6a79cd615968508e56f39660 https://doi.org/10.1101/gad.7.7b.1377

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Conformational changes of U6 RNA during the spliceosome cycle: an intramolecular helix is essential both for initiating the U4-U6 interaction and for the first step of slicing.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Conformational changes of U6 RNA during the spliceosome cycle: an intramolecular helix is essential both for initiating the U4-U6 interaction and for the first step of slicing.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider