Key result
Ewing sarcoma cells secrete EWS/Fli-1 fusion mRNA via microvesicles, which was detectable in approximately 40% of blood samples from mice bearing Ewing sarcoma xenografts.
Ewing sarcoma cells secrete EWS/Fli-1 fusion mRNA via microvesicles, which can be detected in the blood of xenografted mice, indicating potential as a non-invasive diagnostic biomarker.
Hypothesis-generating for Ewing sarcoma liquid biopsy; leaves open human validation before any clinical consideration.
Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was validated that cells emit a heterogeneous mixture of vesicular, organelle-like structures (microvesicles, MVs) into their surroundings including blood and body fluids, and that these MVs contain a selected set of tumor-related proteins and high levels of mRNAs and miRNAs. In this present study, we detected the Ewing sarcoma-specific EWS/Fli-1 mRNA in MVs from the culture medium of ES cell lines carrying t(11;22) (q24; 12). Also, we detected this fusion gene in approximately 40% of the blood samples from mice inoculated with xenografts of TC135 or A673 cells. These findings indicate the EWS/Fli-1 mRNA in MVs might be a new non-invasive diagnostic marker for specific cases of Ewing sarcoma.
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Tsugita et al. (2013) studied Ewing Sarcoma (n=27). Ewing sarcoma cell xenograft (TC135 or A673) vs. Uninoculated control mice was evaluated on Detection of EWS/Fli-1 mRNA in blood-derived microvesicles. Ewing sarcoma cells secrete EWS/Fli-1 fusion mRNA via microvesicles, which was detectable in approximately 40% of blood samples from mice bearing Ewing sarcoma xenografts.
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