What question did this study set out to answer?

To examine the vulnerability of the citric acid cycle enzyme aconitase to oxygen poisoning and assess its role in hyperoxia-induced cellular and lung damage.

December 6, 1994Proceedings of the National Academy of SciencesOpen Access

Aconitase is a sensitive and critical target of oxygen poisoning in cultured mammalian cells and in rat lungs.

View Full Paper

Ask AI

Bookmark

Authors

PGPaul R. GardnerCincinnati Children's Hospital Medical Center DNDiana NguyenTulsa Community College CWCarl W. WhiteNew Jersey Institute of Technology

Discussion

Member takes

Implication

Controlled experimental study demonstrates hyperoxia inactivates aconitase in epithelial cells and rat lungs, indicating disrupted energy metabolism drives oxygen toxicity.

Key Points

To examine the vulnerability of the citric acid cycle enzyme aconitase to oxygen poisoning and assess its role in hyperoxia-induced cellular and lung damage.
Assayed aconitase activity and mitochondrial respiration in cultured human A549 lung epithelial cells exposed to a PO2 of 600 mmHg for 24 hours, with comparisons to fluoroacetate treatment.
Measured total lung aconitase activity in rats following 24-hour exposures to atmospheric PO2 levels of 635 mmHg and 760 mmHg.
Cultured A549 cells exhibited a rapid, progressive loss of > 80% of aconitase activity over 24 hours at 600 mmHg PO2, closely correlating with diminished mitochondrial respiratory capacity.
Rats exposed to 760 mmHg and 635 mmHg PO2 for 24 hours showed 73% and 61% reductions in total lung aconitase activity, respectively.

Cite This Study

Gardner et al. (1994) studied this question.

synapsesocial.com/papers/6a79d3efe9a465269f0db9bb https://doi.org/10.1073/pnas.91.25.12248

View Full Paper

Ask AI

Bookmark

Implication

Controlled experimental study demonstrates hyperoxia inactivates aconitase in epithelial cells and rat lungs, indicating disrupted energy metabolism drives oxygen toxicity.

Aconitase is a sensitive and critical target of oxygen poisoning in cultured mammalian cells and in rat lungs.

Discussion

Member takes

Implication

Key Points

Cite This Study

Aconitase is a sensitive and critical target of oxygen poisoning in cultured mammalian cells and in rat lungs.

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider