Key result
Codon optimization and screening with soluble fusion tags yielded 112 mg/L of soluble functional human ciliary neurotrophic factor in E. coli, a 10-20 fold increase over previous reports.
Population
Escherichia coli strains (BL21pLysS and Rosetta 2pLysS) used for recombinant expression of human ciliary…
Comparison
Codon optimization of hCNTF sequence and… vs Wild type hCNTF sequence and earlier published…
Design
Preclinical
Authors
Loading...
May enable scalable CNTF supply for preclinical research; leaves open clinical translation in cardiovascular disease.
Codon optimization combined with soluble fusion tag screening enables high-yielding, soluble expression of functional human ciliary neurotrophic factor in E. coli without the need for refolding from inclusion bodies.
Itkonen et al. (2014) studied this question. Codon optimization and soluble fusion tags vs. Wild type sequence / previous reports was evaluated on Soluble expression yield of hCNTF. Codon optimization and screening with soluble fusion tags yielded 112 mg/L of soluble functional human ciliary neurotrophic factor in E. coli, a 10-20 fold increase over previous reports.
Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context: