What question did this study set out to answer?

Determine the functional role of MHC class II molecules in the presentation and T-cell receptor recognition of the Mtv-9-encoded retroviral superantigen vSAG-9.

July 1, 1992The Journal of Experimental MedicineOpen Access

Major histocompatibility complex-restricted recognition of retroviral superantigens by V beta 17+ T cells.

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Authors

MBMarcia A. BlackmanTrudeau Institute FLFrances E. LundUniversity of Alabama at Birmingham SSSherri L. SurmanSt. Jude Children's Research Hospital

Discussion

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Implication

In vitro experimental study demonstrates allele-specific MHC class II restriction of retroviral superantigen recognition by T-cell hybridomas, suggesting direct MHC involvement in TCR binding.

Key Points

Determine the functional role of MHC class II molecules in the presentation and T-cell receptor recognition of the Mtv-9-encoded retroviral superantigen vSAG-9.
Assayed a panel of nine V beta 17+ T-cell hybridomas for reactivity against transfected target cell lines.
Presented the retroviral superantigen vSAG-9 using transfected A20/2J (H-2d) and LBK (H-2a) antigen-presenting cells.
Hybridomas exhibited distinct MHC restriction patterns: some recognized vSAG-9 exclusively in the context of H-2a, others exclusively with H-2d, and some responded in the presence of both haplotypes.
Identified that MHC class II molecules actively participate in T-cell receptor recognition rather than acting solely as passive presentation platforms.
Indicated that T-cell receptor domains beyond the V beta region contribute to the specific trimolecular interaction between TCR, superantigen, and MHC class II.

Cite This Study

Blackman et al. (1992) studied this question.

synapsesocial.com/papers/6a79ff93a861e9f599326389 https://doi.org/10.1084/jem.176.1.275

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July 1, 1992The Journal of Experimental MedicineOpen Access

Major histocompatibility complex-restricted recognition of retroviral superantigens by V beta 17+ T cells.

View Full Paper

Ask AI

Bookmark

Authors

MBMarcia A. BlackmanTrudeau Institute FLFrances E. LundUniversity of Alabama at Birmingham SSSherri L. SurmanSt. Jude Children's Research Hospital

Discussion

Member takes

Implication

In vitro experimental study demonstrates allele-specific MHC class II restriction of retroviral superantigen recognition by T-cell hybridomas, suggesting direct MHC involvement in TCR binding.

Key Points

Determine the functional role of MHC class II molecules in the presentation and T-cell receptor recognition of the Mtv-9-encoded retroviral superantigen vSAG-9.
Assayed a panel of nine V beta 17+ T-cell hybridomas for reactivity against transfected target cell lines.
Presented the retroviral superantigen vSAG-9 using transfected A20/2J (H-2d) and LBK (H-2a) antigen-presenting cells.
Hybridomas exhibited distinct MHC restriction patterns: some recognized vSAG-9 exclusively in the context of H-2a, others exclusively with H-2d, and some responded in the presence of both haplotypes.
Identified that MHC class II molecules actively participate in T-cell receptor recognition rather than acting solely as passive presentation platforms.
Indicated that T-cell receptor domains beyond the V beta region contribute to the specific trimolecular interaction between TCR, superantigen, and MHC class II.

Cite This Study

Blackman et al. (1992) studied this question.

synapsesocial.com/papers/6a79ff93a861e9f599326389 https://doi.org/10.1084/jem.176.1.275

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