What question did this study set out to answer?

To determine whether fatty acyl-CoA esters complexed with acyl-CoA-binding protein can modulate ryanodine receptor/Ca2+ release channel activity in skeletal muscle terminal cisternae.

July 15, 1997Biochemical JournalOpen Access

Fatty acyl-CoA–acyl-CoA-binding protein complexes activate the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum

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Authors

RFRosella FulceriUniversity of Siena JKJens KnudsenUniversity of Southern Denmark RGRoberta GiuntiUniversity of Siena

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Implication

Experimental study demonstrates that acyl-CoA–ACBP complexes stimulate calcium release channels in rabbit skeletal muscle, suggesting a lipid-mediated pathway for regulating muscle contraction.

Key Points

To determine whether fatty acyl-CoA esters complexed with acyl-CoA-binding protein can modulate ryanodine receptor/Ca2+ release channel activity in skeletal muscle terminal cisternae.
Assayed Ca2+ efflux and caffeine-induced Ca2+ release in isolated rabbit skeletal muscle terminal cisternae preloaded with Ca2+.
Tested complexes of palmitoyl-CoA (PCoA) or a non-hydrolysable analogue with bovine, rat, or yeast ACBP across varying complex concentrations, [PCoA]-to-[ACBP] ratios, and free [Mg2+] levels.
PCoA bound to excess bovine ACBP stimulated discrete Ca2+ efflux and sensitized channels to sub-optimal caffeine at free PCoA concentrations ≤ 50 nM, with both actions blocked by elevated free [Mg2+].
Rat ACBP complexes (KD ≈ 8 nM) mimicked bovine ACBP complexes (KD ≈ 2 nM), whereas yeast ACBP complexes (KD ≈ 2 nM) displayed greater potency, and non-hydrolysable PCoA analogues similarly sensitized channel opening.

Cite This Study

Fulceri et al. (1997) studied this question.

synapsesocial.com/papers/6a7a00ede357fbdac589515e https://doi.org/10.1042/bj3250423

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Implication

Experimental study demonstrates that acyl-CoA–ACBP complexes stimulate calcium release channels in rabbit skeletal muscle, suggesting a lipid-mediated pathway for regulating muscle contraction.

Fatty acyl-CoA–acyl-CoA-binding protein complexes activate the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum

Discussion

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Implication

Key Points

Cite This Study

Fatty acyl-CoA–acyl-CoA-binding protein complexes activate the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum

Discussion

Member takes

Implication

Key Points

Cite This Study

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