New ex vivo method to objectively assess insulin spatial subcutaneous dispersion through time during pump basal-rate based administration

Figures

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  Figure 1. Experimental setting. 1: Micro-CT scanner, 2: Scanning chamber, 3: External devices chamber, 4: CSII catheter, 5: Microfluidic pressure sensors, 6: Lead protection, 7: Data collection wiring, 8: Computer, 9: Insulin pump.

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  Figure 2. 2D and 3D images of infused explants, with infused insulin and contrast agent numerically identified in green. (a) hypodermal infusion, (b) intradermal infusion, (c) extradermal infusion.

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  Figure 3. 2D slices of one infused explant. Column 1: slices of the explant before infusion was launched. Column 2: corresponding slices after 3 h of insulin infusion. Column 3: corresponding slices after 3 h of infusion, where the insulin and contrast agent mixture detected by the algorithm was numerically identified in green.

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  Figure 4. Cannula content during 3 h of infusion, during a test where an occlusion occurred.

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  Figure 5. Index of dispersion (IoD) evolution through time, with mean and standard deviation. (a) All infusions (N = 21), (b) Infusions reaching hypodermis (N = 13), (c) Infusions reaching dermis/hypodermis limit (N = 3), (d) Infusions reaching dermis (N = 4), (e) Extradermal infusion (N = 1), (f) All infusions, aggregated by donor (N = 21).

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  Figure 6. Boxplot of IoD values after 3 h of infusion presenting minimas, maximas, quartiles and median. Classified according to the tissue structure reached by the cannula.

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  Figure 7. Pressure record and corresponding cannula content for one injection.