What question did this study set out to answer?

Design, synthesize, and functionally evaluate a 770-kilobase synthetic yeast chromosome II (synII) to examine the biological effects of genome-scale modifications.

March 10, 2017ScienceOpen Access

Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

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Authors

YSYue ShenJiangxi Science and Technology Normal University YWYun WangBGI Group (China)YWYun WangBGI Group (China)

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Implication

Experimental study demonstrates near-native viability and targeted debugging of synthetic chromosome synII in yeast, indicating the feasibility of full-scale eukaryotic genome redesign.

Key Points

Design, synthesize, and functionally evaluate a 770-kilobase synthetic yeast chromosome II (synII) to examine the biological effects of genome-scale modifications.
Constructed synII in vivo using an I-SceI endonuclease-mediated cleavage strategy to accelerate targeted homologous recombination across synthetic chromosome arms.
Characterized chromosome stability, replication, and biological processes using multi-omics platforms, including transcriptomics, proteomics, metabolomics, and phenomics.
Identified and repaired growth defects using wild-type sequence complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution).
The I-SceI cleavage strategy shortened synthetic arm integration time by 50% and produced a tenfold increase in URA3-deficient recombinant colonies.
SynII replicated, segregated, and supported cell growth similarly to wild-type yeast, though deletion of 13 tRNA genes induced a modest upregulation of the translational machinery.
A conditional growth defect at 37°C in glycerol medium was mapped to recoding in TSC10 within the high-osmolarity glycerol pathway and rescued by restoring wild-type TSC10.

Cite This Study

Shen et al. (2017) studied this question.

synapsesocial.com/papers/6a7a03351ba4614d7ff3bea1 https://doi.org/10.1126/science.aaf4791

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Implication

Experimental study demonstrates near-native viability and targeted debugging of synthetic chromosome synII in yeast, indicating the feasibility of full-scale eukaryotic genome redesign.

Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

Discussion

Member takes

Implication

Key Points

Cite This Study

Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

Discussion

Member takes

Implication

Key Points

Cite This Study

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