Key result
IL-6 preconditioning protected neonatal rat ventricular cardiomyocytes against H2O2-induced cell death by upregulating prohibitin expression through STAT3 phosphorylation.
IL-6 preconditioning protects neonatal rat cardiomyocytes from oxidative stress-induced death by upregulating prohibitin via STAT3 phosphorylation.
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No clinical translation yet from neonatal rat cells; leaves open in vivo and human relevance of IL-6/STAT3 protection.
OBJECTIVE: Hydrogen peroxide (H(2)O(2)) is a potent reactive oxygen species that causes cardiomyocytes injury. As an important cytokine, interleukin 6 (IL-6) has cardioprotective effects as it plays an essential role in the late phase of preconditioning. Our work is to investigate if IL-6 preconditioning has protective effects on neonatal rat ventricular cardiomyocytes in response to H(2)O(2) and its underlying mechanism. METHODS: Gel-based comparative proteomic approach along with small interfering RNA (siRNA) and Western blot analysis was used to analyse mechanisms of IL-6 preconditioning on H(2)O(2)-induced neonatal rat ventricular cardiomyocytes injury. RESULTS: IL-6 preconditioning protected cardiomyocytes against H(2)O(2)-induced cell death. Proteomic analysis showed that IL-6 pretreatment further increased the expression of prohibitin and improved the viability of cardiomyocytes exposed to H(2)O(2). Knocking down of prohibitin with siRNA abrogated this protection by increasing apoptosis rate. Tyrosine kinase inhibitor AG490 decreased signal transducers and activators of transcription 3 (STAT3) phosphorylation and down-regulated prohibitin expression in cardiomyocytes pretreated with IL-6 and exposed to H(2)O(2), which further dampened the protective effects of IL-6 preconditioning. CONCLUSION: Our results provide direct evidence that prohibitin is a protective factor of IL-6 preconditioning in H(2)O(2)-induced neonatal rat ventricular cardiomyocytes death. The upregulation of prohibitin by IL-6 is, at least, partially regulated through STAT3 phosphorylation.
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Jia et al. (2012) studied H2O2-induced cardiomyocytes injury. IL-6 preconditioning vs. No preconditioning / H2O2 alone was evaluated on Cell death and prohibitin expression. IL-6 preconditioning protected neonatal rat ventricular cardiomyocytes against H2O2-induced cell death by upregulating prohibitin expression through STAT3 phosphorylation.
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