Key result
Using permeabilized muscle fibers (PMT) instead of isolated mitochondria provides a reference method for quantifying mitochondrial ischemia-reperfusion damage in porcine and human tissue.
Why the study?
Methods to quantify mitochondrial function in translationally relevant models and in human tissue are needed to improve the translation of cardioprotection to patients.
The use of permeabilized muscle tissue provides a translationally relevant method for quantifying mitochondrial ischemia-reperfusion damage in cardiac specimens.
PMT-based mitochondrial assessment enables studies in human and large-animal tissue; leaves open clinical translation of cardioprotection.
Methods to quantify mitochondrial function in translationally relevant models and in human tissue are needed to improve the translation of cardioprotection to patients. Our present approach, using PMT instead of isolated mitochondria for the quantification of mitochondrial ischemia-reperfusion damage, provides a reference for further studies in translationally relevant large animal models and in human tissue, thus possibly improving the translation of cardioprotection to the benefit of patients with acute myocardial infarction.
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Eickelmann et al. (2023) studied Ischemia-reperfusion damage. Permeabilized muscle fibers (PMT) vs. Isolated mitochondria was evaluated on Quantification of mitochondrial ischemia-reperfusion damage. Using permeabilized muscle fibers (PMT) instead of isolated mitochondria provides a reference method for quantifying mitochondrial ischemia-reperfusion damage in porcine and human tissue.
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