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Myelin osmiophilia has been shown to develop significantly later than myelin staining by Luxol fast blue and Sudan black, in the developing kitten optic nerve. These histological changes are accompanied by alterations in the lipid composition of the optic nerve. Although myelination commences at about 10 days post partum in the nerve the appearance of cerebrosides is unexpectedly delayed. Changes in fatty acid chain length and lipid composition of optic nerve are consistent with the suggestion that ‘early’ myelin may be unchanged glial plasma membrane.
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Banik et al. (1968) studied this question.
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