What question did this study set out to answer?

To define stemness-related molecular subtypes in ovarian cancer and characterize their immune features, prognostic significance, and therapeutic targets.

June 11, 2025Frontiers in OncologyOpen Access

Stemness-driven clusters in ovarian cancer: immune characteristics and prognostic implications

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To define stemness-related molecular subtypes in ovarian cancer and characterize their immune features, prognostic significance, and therapeutic targets.
Compared transcriptomic profiles between adherent and sphere-forming SKOV3 ovarian cancer cells to identify stemness-associated genes.
Constructed a prognostic risk model and nomogram using unsupervised clustering, WGCNA, and LASSO regression across ovarian cancer patient cohorts.
Profiled the tumor microenvironment with single-cell RNA sequencing and evaluated AKAP12 function through cellular knockdown assays, immunohistochemistry, and organoid models.
Unsupervised clustering identified three stemness-related subgroups exhibiting distinct survival outcomes, tumor mutation burdens, and immune checkpoint profiles.
Single-cell analysis pinpointed fibroblasts as the primary cellular component driving and maintaining stemness traits in the tumor microenvironment.
AKAP12 expression positively tracked with stemness phenotypes and OX40L, while AKAP12 knockdown suppressed sphere formation, restricted cell migration, and restored cisplatin sensitivity.

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Implication

Translational study reveals stemness-driven molecular subtypes and identifies AKAP12 as a therapeutic target in ovarian cancer, suggesting avenues for tailored immunotherapy.

Cite This Study

Zeng et al. (2025) studied this question.

synapsesocial.com/papers/6a7a347f14b028facf0e37f1 https://doi.org/10.3389/fonc.2025.1577283

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Implication

Translational study reveals stemness-driven molecular subtypes and identifies AKAP12 as a therapeutic target in ovarian cancer, suggesting avenues for tailored immunotherapy.

Stemness-driven clusters in ovarian cancer: immune characteristics and prognostic implications

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Discussion

Implication

Cite This Study

Stemness-driven clusters in ovarian cancer: immune characteristics and prognostic implications

Key Points

Discussion

Implication

Cite This Study

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