What question did this study set out to answer?

To determine whether monocyte chemoattractant protein-1 (MCP-1) regulates intercellular adhesion molecule-1 (ICAM-1) expression on cardiac myocytes and mediates neutrophil adhesion in vitro.

August 1, 1994Cardiovascular Research

Expression of intercellular adhesion molecule-1 on rat cardiac myocytes by monocyte chemoattractant protein-1

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To determine whether monocyte chemoattractant protein-1 (MCP-1) regulates intercellular adhesion molecule-1 (ICAM-1) expression on cardiac myocytes and mediates neutrophil adhesion in vitro.
Measured MCP-1/JE and ICAM-1 mRNA levels in cultured neonatal rat cardiac myocytes using Northern blot analysis under exposure to interleukin-1 alpha, lipopolysaccharide, or hypoxia.
Quantified surface ICAM-1 protein expression on myocytes via ELISA following stimulation with MCP-1.
Assessed rat neutrophil adhesion to cardiac myocytes in co-incubation assays in the presence of MCP-1 and an anti-ICAM-1 monoclonal antibody.
MCP-1/JE mRNA was undetectable at baseline but was induced by exposure to interleukin-1 alpha (100 U/ml), lipopolysaccharide (1 µg/ml), or hypoxia.
MCP-1 increased ICAM-1 mRNA with peak elevation at 2 hours and significantly stimulated ICAM-1 protein levels in a dose-dependent manner beginning at 6 hours.
MCP-1 enhanced neutrophil adhesion to cardiac myocytes, an effect that was specifically blocked by anti-ICAM-1 monoclonal antibodies.

Structured PICO

Population

Cultured neonatal rat cardiac myocytes and rat neutrophils

Intervention

Exposure to monocyte chemoattractant protein-1 (MCP-1), interleukin 1 alpha (100 U.ml-1), lipopolysaccharide (1 microgram.ml-1), or hypoxia (95% N2 + 5% CO2)

Comparator

Unstimulated control conditions

Outcome

MCP-1/JE and ICAM-1 mRNA expression, ICAM-1 molecule content, and neutrophil-myocyte adhesionsurrogate

MCP-1 produced by cardiac myocytes promotes neutrophil adhesion via ICAM-1 expression, suggesting a mechanism for cardiac inflammation in acute myocardial infarction and myocarditis.

Discussion

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Implication

May mediate neutrophil-myocyte adhesion in cardiac inflammation; hypothesis-generating for MI or myocarditis, needs in vivo confirmation.

Cite This Study

Ban et al. (1994) studied this question.

synapsesocial.com/papers/6a7a37a7e357fbdac58969ee https://doi.org/10.1093/cvr/28.8.1258

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Expression of intercellular adhesion molecule-1 on rat cardiac myocytes by monocyte chemoattractant protein-1

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Expression of intercellular adhesion molecule-1 on rat cardiac myocytes by monocyte chemoattractant protein-1

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider