Microbes in thawing permafrost: the unknown variable in the climate change equation

Considering that 25% of Earth's terrestrial surface is underlain by permafrost (ground that has been continuously frozen for at least 2 years), our understanding of the diversity of microbial life in this extreme habitat is surprisingly limited. Taking into account the total mass of perennially frozen sediment (up to several hundred meters deep), permafrost contains a huge amount of buried, ancient organic carbon ( Tarnocai et al., 2009 ). In addition, permafrost is warming rapidly in response to global climate change ( Romanovsky et al., 2010 ), potentially leading to widespread thaw and a larger, seasonally thawed soil active layer. This concern has prompted the question: will permafrost thawing lead to the release of massive amounts of carbon dioxide (CO 2 ) and methane (CH 4 ) into the atmosphere? This question can only be answered by understanding how the microbes residing in permafrost will respond to thaw, through processes such as respiration, fermentation, methanogenesis and CH 4 oxidation ( Schuur et al., 2009 ).