Arp2/3 complex–dependent actin networks constrain myosin II function in driving retrograde actin flow
Key result
Inhibition of the Arp2/3 complex increased retrograde actin flow rates, but when myosin II activity was blocked, Arp2/3 complex inhibition slowed retrograde actin flow and prevented veil retraction.
Population
Neuronal growth cones
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Arp2/3-myosin II balance shapes growth cone motility; leaves open relevance to cardiovascular cell migration or remodeling.
Structured PICO
P
Population
Neuronal growth cones
I
Intervention
Inhibition of the Arp2/3 complex with CK666 and/or myosin II with small molecules
O
Outcome
Retrograde actin flow rates and actin veil dynamicssurrogate
Actin networks assembled by the Arp2/3 complex restrict myosin II-dependent contractility, affecting growth cone motility.
Cite This Study
Yang et al. (2012) studied Neuronal growth cone actin dynamics. Inhibition of Arp2/3 complex with CK666 and/or myosin II was evaluated on Retrograde actin flow rates and veil retraction. Inhibition of the Arp2/3 complex increased retrograde actin flow rates, but when myosin II activity was blocked, Arp2/3 complex inhibition slowed retrograde actin flow and prevented veil retraction.