Why the study?
Does RT-PCR detect human metapneumovirus in middle ear specimens from children with acute otitis media?
Does RT-PCR detect human metapneumovirus in middle ear specimens from children with acute otitis media?
Human metapneumovirus can be detected in a small proportion of middle ear specimens from children with acute otitis media.
Low hMPV detection in AOM middle ear fluid suggests limited role; leaves open its etiologic contribution and need for prospective studies.
Nasal and middle ear specimens collected from children with acute otitis media were subjected to viral isolation and bacteria culture. All virus-negative specimens underwent reverse transcription polymerase chain reaction to detect human metapneumovirus. Three of 126 middle ear specimens were positive by this assay.
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Suzuki et al. (2005) studied this question.
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